Detection of multiple virulence-associated genes of Listeria monocytogenes by PCR in artificially contaminated milk samples.

نویسندگان

  • K J Cooray
  • T Nishibori
  • H Xiong
  • T Matsuyama
  • M Fujita
  • M Mitsuyama
چکیده

The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 60 8  شماره 

صفحات  -

تاریخ انتشار 1994